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avidin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin
    Avidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AK-5004/VECTASTAIN+ABC-AP+Staining+KIT+(Alkaline+Phosphatase%2C+Rat+IgG)/pmc11209741-116-12-20
    Average 92 stars, based on 24 article reviews
    avidin - by Bioz Stars, 2026-10
    92/100 stars

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    Incubation:

    Article Title: Exploring the effects of taurolidine on tumor weight and microvessel density in a murine model of osteosarcoma
    Article Snippet: Sections were washed three times in PBS-Tween 1% for 5 min and incubated with the biotinylated secondary mouse anti-rat antibody (Dako, USA; 1:200 dilution in Tris-HCL buffer) for 30 min. .. Sections were washed three times with PBST and incubated in previously prepared Avidin/Biotinylated enzyme complex (ABC-Vectastain AK 5004; ratio 1:1; Vector Laboratories Inc., USA; 1:50 dilution in PBS) for 30 min. Incubation was terminated with three washing steps in PBST. .. Finally, the sections were incubated with the enzyme substrate Fast Red (Dako, USA) for 30 min for CD31 staining and 18 min for BrdU staining both determined with doubly distilled water.

    Avidin-Biotin Assay:

    Article Title: Exploring the effects of taurolidine on tumor weight and microvessel density in a murine model of osteosarcoma
    Article Snippet: Sections were washed three times in PBS-Tween 1% for 5 min and incubated with the biotinylated secondary mouse anti-rat antibody (Dako, USA; 1:200 dilution in Tris-HCL buffer) for 30 min. .. Sections were washed three times with PBST and incubated in previously prepared Avidin/Biotinylated enzyme complex (ABC-Vectastain AK 5004; ratio 1:1; Vector Laboratories Inc., USA; 1:50 dilution in PBS) for 30 min. Incubation was terminated with three washing steps in PBST. .. Finally, the sections were incubated with the enzyme substrate Fast Red (Dako, USA) for 30 min for CD31 staining and 18 min for BrdU staining both determined with doubly distilled water.

    Article Title: Endogenous Retrovirus Gag Antigen and Its Gene Variants Are Unique Autoantigens Expressed in the Pancreatic Islets of Non-obese Diabetic Mice
    Article Snippet: The primary antibodies used in this study were rat anti-Gag mAb R187 ( 33 ), Biotin-labelled anti-CD105 (clone MJ7/18, BioLegend) and Biotin-labelled anti-Sca-1 (clone D7, BioLegend). .. The secondary antibodies used were anti-rat Ig-HRP (Cat#A10549, Invitrogen), AP-labelled secondary reagent for detecting rat IgG (AK5004, Vector Lab), and Avidin-HRP (Cat#RPN1231V, GE Healthcare). ..

    Article Title: Endogenous retrovirus Gag antigen and its gene variants are unique autoantigens expressed in the pancreatic islets of non-obese diabetic mice.
    Article Snippet: The primary antibodies used in this study were rat anti-Gag mAb R187 [33], Biotin-labelled anti-CD105 (clone MJ7/18, BioLegend) and Biotin-labelled anti-Sca-1 (clone D7, BioLegend). .. The secondary antibodies used were anti-rat IgHRP (Cat#A10549, Invitrogen), AP-labelled secondary reagent for detecting rat IgG (AK5004, Vector Lab), and Avidin-HRP (Cat#RPN1231 V, GE Healthcare). ..



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